western blot technique meaning in Chinese

西式印迹技术:分析和鉴定蛋白抗原的技术方法

To investigate the potential function of nogo - a involved in neuronal development and differentiation , this study mainly used hippocampal neuron culture model , both in conventional and low - density condition , and immunohistochernical and western blot techniques . nogo - a expression pattern in hippocampal neurons at different stages and its subcellular distribution were explored by using specific antibody raised against nogo - a . pc12 , 3t3 cells were also cultured as controls
为探讨nogo a在神经元中的表达与神经元发育分化的关系，本研究借助了海马神经元常规培养方法和低密度条件下培养方法，应用针对nogo a的特异性抗体，采用免疫荧光细胞化学染色和westernblot方法，观察了nogo a在体外培养的大鼠海马神经元表达分布模式，和不同培养时间nogo a亚细胞分布的变化。